GE17-0980-01
Butyl Sepharose™ 4 Fast Flow
Cytiva 17-0980-01, pack of 200 mL
Sinonimo/i:
Butyl Sepharose, Fast Flow Sepharose
About This Item
Prodotti consigliati
ligand
butyl
Confezionamento
pack of 200 mL
Produttore/marchio commerciale
Cytiva 17-0980-01
Matrice
4% cross-linked agarose
Dimensione particelle
45-165 μm
Diametro medio
90 μm
cleaning in place
2-14
Intervallo di esercizio
3-13
Compatibilità
suitable for bioprocess medium
Categorie correlate
Descrizione generale
Applicazioni
Butyl Sepharose™ 4 Fast FLow is based on cross-linked 4% Agarose. The aliphatic ligand is immobilized to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.
Butyl Sepharose™ 4 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Butyl Sepharose™ 4 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Caratteristiche e vantaggi
- Standard aliphatic HIC media based on Sepharose™ Fast FLow base matrix derivatized via uncharged, chemically-stable ether linkages.
- Optimized for the separation of larger Proteins in capture and intermediate purification steps
- BioProcess medium supported for industrial applications and well-established in approved processes
- The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.
Stoccaggio e stabilità
Risultati analitici
Note legali
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Codice della classe di stoccaggio
3 - Flammable liquids
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Articoli
This section is about salt selection and buffer preparation in HIC medium from GE Healthcare, parameters such as buffer ions and pH are considered
This page discusses column packing and preparation techniques for reverse phase chromatography
Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with GE Healthcare media are detailed, including sample application, load volume and temperature
Protocolli
While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent cleaning conditions and their low level of non-specific interaction.
This page discusses various aspects of sample preparation for chromatographic purification.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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