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G9136

Sigma-Aldrich

Porcine Gelatin

from porcine skin, Type A, powder, γ-irradiated, suitable for cell culture, BioXtra

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About This Item

Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
12352202
NACRES:
NA.75

Nome del prodotto

Gelatina, Type A, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

Origine biologica

Porcine skin

Livello qualitativo

Sterilità

γ-irradiated

Tipo

Type A

Nome Commerciale

BioXtra

Stato

lyophilized powder

Confezionamento

pkg of 10 mg

tecniche

cell culture | mammalian: suitable

Copertura della superficie

100‑200 μg/cm2

Resistenza del gel

~300 g Bloom

Solubilità

soluble 20 mg/mL, clear, colorless (in warm tissue culture medium)

Condizioni di spedizione

ambient

Temperatura di conservazione

room temp

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Applicazioni

This product is recommended for use as a cell culture substratum at 100-200 μg/cm2. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Componenti

La gelatina è una miscela eterogenea di proteine solubili in acqua ad alta massa molecolare media, presente nel collagene. Le proteine si estraggono facendo bollire in acqua pelle, tendini, legamenti, ossa, ecc. adatti. La gelatina di tipo A deriva da tessuto trattato con acidi. Quella di tipo B da tessuto trattato con calce.

Avvertenza

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Nota sulla preparazione

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g.

This product is a type A lyophilized powder, suitable for cell culture, with a gel strength of 300.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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