48724

Gelatina

suitable for microbiology, high gel strength

• Numero CAS: 9000-70-8
• Numero CE: 232-554-6
• Numero MDL: MFCD00081638
• Codice UNSPSC: 41106212
• NACRES: NA.85

Proprietà

• Origine biologica: Porcine
• Livello qualitativo: 100
• Sterilità: non-sterile
• Forma fisica: powder
• Qualità: high gel strength
• Durata: limited shelf life, expiry date on the label
• Residuo alla calcinazione: ≤2%
• Perdita: ≤15% loss on drying
• Trasmittanza: 450 nm, ≥85%; 620 nm, ≥95%
• pH: 4.0-6.0 (25 °C, 67 mg/mL in H₂O); 5.20-5.60
• Viscosità: 5.10-5.80 mPa.s
• Resistenza del gel: 245-275 g Bloom (67 mg/ml water); 280-320 g BloomAOAC
• Solubilità: H₂O: 67 mg/mL at 50 °C, slightly hazy, faintly yellow; H₂O: 67 mg/mL at 60 °C
• Anioni in tracce: chloride (Cl^-): ≤2000 mg/kg
• Cationi in tracce: Ca: ≤2000 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤10 mg/kg; Cu: ≤50 mg/kg; Fe: ≤50 mg/kg; K: ≤500 mg/kg; Mg: ≤500 mg/kg; Mn: ≤5 mg/kg; Na: ≤1200 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Zn: ≤10 mg/kg
• applicazioni: microbiology

Descrizione

Applicazioni

This product is recommended for use as a cell culture substratum at 1-5 μg/cm² or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Componenti

La gelatina è una miscela eterogenea di proteine solubili in acqua ad alta massa molecolare media, presente nel collagene. Le proteine si estraggono facendo bollire in acqua pelle, tendini, legamenti, ossa, ecc. adatti. La gelatina di tipo A deriva da tessuto trattato con acidi. Quella di tipo B da tessuto trattato con calce.

Avvertenza

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Nota sulla preparazione

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 250. Manufactured by Gelita AG

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)