FG0465

IMDM

With stable-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

• Sinonimo/i: Cell culture medium, Iscove′s MDM, Iscove’s Modified Dulbecco’s Medium, Modified Dulbecco′s medium
• Codice UNSPSC: 12352207
• NACRES: NA.71

Proprietà

• product name: Iscove′s Modified Dulbecco′s Medium, With L-alanyl-L-glutamine and sodium bicarbonate, sterile-filtered, liquid, suitable for cell culture
• Livello qualitativo: 400
• Sterilità: sterile-filtered; sterile
• Nome Commerciale: AQmedia^™
• Forma fisica: liquid
• tecniche: cell culture | mammalian: suitable
• Impurezze: Endotoxin, tested
• Componenti: stable glutamine: yes; NaHCO₃: yes; glucose: yes; phenol red: yes
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Applicazioni

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco¢s Modified Eagle¢s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove¢s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Note legali

AQmedia is a trademark of Sigma-Aldrich Co. LLC

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable