F1763
Anti-Rat IgG (whole molecule)–FITC antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Sinonimo/i:
Rabbit Anti-Rat IgG (whole molecule)–Fluorescein isothiocyanate
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About This Item
Prodotti consigliati
Origine biologica
rabbit
Coniugato
FITC conjugate
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
secondary antibodies
Clone
polyclonal
Stato
buffered aqueous solution
Condizioni di stoccaggio
protect from light
tecniche
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320
indirect immunofluorescence: 1:320
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
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Descrizione generale
Anti-Rat IgG (whole molecule) is produced in rabbit using purified rat IgG as the immunogen.
Rat IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rat serum. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Anti-Rat IgG (whole molecule)-FITC antibody binds to all rat immunoglobulins.
Specificità
Rabbit polyclonal anti-Rat IgG (whole molecule)−FITC antibody reacts with rat IgG in vitro, in rat serum and biological fluids.
Immunogeno
Purified rat IgG
Applicazioni
Anti-Rat IgG (whole molecule)-FITC antibody has been used:
- in immunolabelling for confocal laser scanning microscopy (CLSM)
- in immunostaining
Anti-Rat IgG (whole molecule)-FITC antibody is suitable for use in flow cytometry (1:100) and immunofluorescence.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)
Rabbit polyclonal anti-Rat IgG (whole molecule)-FITC antibody may be used to detect and quantitate the level of IgG in rat serum and biological fluids by fluorescent techniques. It may also be used as a secondary antibody in assays that use pig IgG as the primary antibody.
Azioni biochim/fisiol
IgGs are glycoprotein antibodies that modulate several immune responses. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins.
Altre note
Antibody adsorbed with human IgG.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Nota sulla preparazione
Adsorbed to reduce background staining with human samples.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Dispositivi di protezione individuale
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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