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D9443

SAFC

Dulbecco′s Modified Eagle′s Medium - low glucose

liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling in cell culture applications

Sinonimo/i:

DME, DMEM

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About This Item

Numero MDL:
MFCD00217342
Codice UNSPSC:
12352207
NACRES:
NA.75

Descrizione

With 1000mg/L D-glucose, L-glutamine, and sodium bicarbonate. Without arginine, leucine, lysine, sodium pyruvate, and phenol red

Sterilità

sterile-filtered

Forma fisica

liquid

tecniche

cell culture | mammalian: suitable

Impurezze

endotoxin, tested

Componenti

NaHCO3: 3.7 g/L
sodium pyruvate: no
glucose: 1.0 g/L
L-glutamine: 0.584 g/L
phenol red: no
HEPES: no

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Ricostituzione

Designed for use in stable isotope labeling with amino acids in cell culture applications.
Supplement with 0.100 g/L L-Arginine-13C6, 15N4 HCl (Product 608033), 0.100 g/L L-Leucine-13C6, 15N (Product 608068), and 0.100 g/L L-Lysine-13C6, 15N2 (Product 608041).

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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A M Edick et al.
Journal of dairy science, 104(1), 1123-1135 (2020-11-10)
The objective of this study was to determine the role of GCN2 in the response to AA deprivation of primary bovine mammary epithelial cells (BMEC). Cells were isolated from the mammary tissue of 2 lactating Holstein cows by enzymatic digestion
Mahesh Saqcena et al.
PloS one, 8(8), e74157-e74157 (2013-08-27)
In multicellular organisms, cell division is regulated by growth factors (GFs). In the absence of GFs, cells exit the cell cycle at a site in G1 referred to as the restriction point (R) and enter a state of quiescence known
Sona Hubackova et al.
Molecular oncology, 13(7), 1467-1489 (2019-03-29)
Radiation and chemotherapy represent standard-of-care cancer treatments. However, most patients eventually experience tumour recurrence, treatment failure and metastatic dissemination with fatal consequences. To elucidate the molecular mechanisms of resistance to radio- and chemotherapy, we exposed human cancer cell lines (HeLa
Deven Patel et al.
The Journal of biological chemistry, 292(3), 936-944 (2016-12-14)
Lipids are important nutrients that proliferating cells require to maintain energy homeostasis as well as to build plasma membranes for newly synthesized cells. Previously, we identified nutrient-sensing checkpoints that exist in the latter part of the G1 phase of the
Dipsikha Biswas et al.
The Journal of biological chemistry, 295(46), 15597-15621 (2020-09-04)
Branched-chain α-keto acids (BCKAs) are catabolites of branched-chain amino acids (BCAAs). Intracellular BCKAs are cleared by branched-chain ketoacid dehydrogenase (BCKDH), which is sensitive to inhibitory phosphorylation by BCKD kinase (BCKDK). Accumulation of BCKAs is an indicator of defective BCAA catabolism
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