M3942

DMEM - high glucose

MegaCell^™, with sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell cutlure

• Sinonimo/i: DMEM, DMEM Supplement
• Codice UNSPSC: 12352207
• NACRES: NA.75

Proprietà

• Nome del prodotto: MegaCell^™ Dulbecco′s Modified Eagle′s Medium, liquid, sterile-filtered, suitable for cell culture
• Sterilità: sterile-filtered
• Stato: liquid
• tecniche: cell culture | mammalian: suitable
• Impurezze: endotoxin, tested
• Componenti: L-glutamine: no; glucose: 4500 mg/L (Dextro); sodium pyruvate: yes
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

MegaCell^™ is a multifaceted media developed to reduce the amount of serum required for culturing mammalian cells in vitro. It reduces the supplementation of fetal bovine serum (FBS) or other serum from 10% to 3% and supports proliferative rates and maximal cell densities. MegaCell enables maintaining a long-term culture of non-fastidious cell lines.

Applicazioni

MegaCell^™ Dulbecco′s Modified Eagle′s Medium has been used:

• as a component of the M10 medium to culture CD133⁺ cells from biopsies of para-spinal and quadriceps muscle of Duchenne muscular dystrophy patients
• as a component of the proliferation medium for the expansion and cryopreservation of human induced pluripotent stem (iPS) cells cultured without feeder cells and human embryonic (ES) cell-derived mesoangioblast-like cells
• to culture the positive hybridomas during the production of mouse anti-chicken CD25 monoclonal antibodies

Ricostituzione

Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)

Note legali

Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.

MegaCell is a trademark of Promega Corp.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable