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Key Documents

D4522

Sigma-Aldrich

Deoxyribonucleic acid from calf thymus

Type XV, Activated, lyophilized powder

Sinonimo/i:

Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid

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About This Item

Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
41106310
eCl@ss:
32160414
NACRES:
NA.52

Grado

for molecular biology

Livello qualitativo

Tipo

Type XV

Forma fisica

lyophilized powder

Temperatura di conservazione

−20°C

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Descrizione generale

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Applicazioni

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Caratteristiche e vantaggi

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Quantità

One mg DNA is approx. 20 A260 units.

Nota sulla preparazione

Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.

Ricostituzione

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves


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Claudia Gomes et al.
Life science alliance, 4(11) (2021-09-11)
High levels of autoimmune antibodies are observed in COVID-19 patients but their specific contribution to disease severity and clinical manifestations remains poorly understood. We performed a retrospective study of 115 COVID-19 hospitalized patients with different degrees of severity to analyze
Parul Natvar Patel et al.
Tissue engineering, 11(9-10), 1498-1505 (2005-11-02)
The ultimate goal of this research is to develop an injectable cell-scaffold system capable of permitting adipogenesis to abrogate soft tissue deficiencies resulting from trauma, tumor resection, and congenital abnormalities. The present work compares the efficacy of photopolymerizable poly(ethylene glycol)
Anastasia S Singatulina et al.
Cell reports, 27(6), 1809-1821 (2019-05-09)
PARP-1 synthesizes long poly(ADP-ribose) chains (PAR) at DNA damage sites to recruit DNA repair factors. Among proteins relocated on damaged DNA, the RNA-binding protein FUS is one of the most abundant, raising the issue about its involvement in DNA repair.
Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.
H V APOSHIAN et al.
The Journal of biological chemistry, 237, 519-525 (1962-02-01)
Benjamin D Dickson et al.
Molecules (Basel, Switzerland), 24(8) (2019-04-24)
Poly(ADP-ribose)polymerase (PARP) inhibitors (PARPi) have recently been approved for the treatment of breast and ovarian tumors with defects in homologous recombination repair (HRR). Although it has been demonstrated that PARPi also sensitize HRR competent tumors to cytotoxic chemotherapies or radiotherapy

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