AFRC 17D1

94120825, Application: Fusion partner for hybridoma production

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: Horse x Mouse/Horse Hybridoma
• ImmunoDon: Horse peripheral lymphocytes
• Mieloma: NS0
• Reattività: Application: Fusion partner for hybridoma production
• tecniche: cell culture | mammalian: suitable
• Isotipo: Not specified
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Horse peripheral blood lymphocytes x Mouse NS0

Descrizione della linea cellulare

The heterohybridoma AFRC 17D1 was obtained by fusion of lymphocytes from ponies immunised with the influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)) with mouse myeloma NS0 cells. The hybridoma was made aminopterin-sensitive by selective growth in 8-azaguanine. This non-Ig-secreting cell line was used as fusion partner for the establishment of equine-mouse heterohybidomas.

Immunogeno

Influenza A equine 2 virus

Applicazioni

Fusion partner for hybridoma production

Terreno di coltura

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Mantenimento delle subcolture

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.