MDCK

85011435, dog kidney, Epithelial-like

• Sinonimo/i: MDCK1 Cells, MDCKI Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
• Codice UNSPSC: 41106514
• eCl@ss: 32190102

Proprietà

• product name: MDCK, 85011435
• Origine biologica: dog kidney
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 78
• Morfologia: Epithelial-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Canine Cocker Spaniel kidney

Descrizione della linea cellulare

Derived from the kidney of a normal adult Cocker Spaniel in 1958. Useful in viral studies, supports the growth of vesicular exanthema virus of swine, vesicular stomatitis, vaccina, coxsackie B-5, adenoviruses, reoviruses and infectious canine hepatitis. Also known as MDCK (NBL-2).

Applicazioni

Virus studies: SVEV, VSV, Vaccinia, reoviruses and inf. canine hepatitis virus

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.

Altre note

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