11011375001
Roche
Nutridoma™-SP
suitable for hybridoma, solution, pkg of 100 mL (100x)
Sinonimo/i:
hybridoma, nutridoma
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About This Item
Codice UNSPSC:
12352207
Prodotti consigliati
Sterilità
sterile; 0.2 μm filtered
Livello qualitativo
Stato
solution
Confezionamento
pkg of 100 mL (100x)
Produttore/marchio commerciale
Roche
Concentrazione
100 ×
tecniche
cell culture | hybridoma: suitable
Impurezze
mycoplasma, tested
≤100 EU/mL Endotoxin (LAL test)
Solubilità
water: miscible
Temperatura di conservazione
20-25°C
Categorie correlate
Descrizione generale
Nutridoma is a family of chemically defined supplements that can completely replace serum in cell culture media. Nutridoma-SP is used for the cultivation of established myeloma and hybridoma cells. It is used for the serum-free cultivation of murine myelomas and hybridomas that have an intact cholesterol biosynthesis pathway, such as those derived from the SP2/0, and some of the P3x63Ag8.653 cell lines and lymphoblastoid cell lines, as well as for primary lymphoid cell cultures. It is also used for the serum-free culture of a variety of other cell types, including neural explants.
Applicazioni
Nutridoma-SP has been used in the culture media for canine trophoblasts, human embryonic stem cells and PLB-985 cells.
Definizione di unità
Endotoxin (LAL): 10 EU/ml; mycoplasma-tested
Stato fisico
Solution, 100x concentrated, filtered through 0.2 μm pore size membrane
Biochemically defined serum-free medium supplement composed of albumin, insulin, transferrin, and other defined organic and inorganic compounds. Nutridoma contains no other growth factors, mitogens, hormones, or sterols.
The solution contains the pH indicator phenol red and appears clear.
Biochemically defined serum-free medium supplement composed of albumin, insulin, transferrin, and other defined organic and inorganic compounds. Nutridoma contains no other growth factors, mitogens, hormones, or sterols.
The solution contains the pH indicator phenol red and appears clear.
Nota sulla preparazione
Working concentration: Nutridoma-SP (100x) is diluted 1:100 (v/v) with sterile basal medium. Use high glucose DMEM/RPMI 1640 (1:1) (R/D medium) for Nutridoma-SP. The final medium should also contain L-glutamine and sodium bicarbonate. The protein concentration is less than 50 μg/ml for 1% working concentration.
Storage conditions (working solution): 2 to 8 °C
Nutridoma working solutions, e.g., media containing 1% Nutridoma (1%) are stable for 4 weeks when stored at 2 to 8 °C in the dark. Do not freeze.
Note: Do not store in plastic as adsorption of ingredients to the plastic surface may occur.
Storage conditions (working solution): 2 to 8 °C
Nutridoma working solutions, e.g., media containing 1% Nutridoma (1%) are stable for 4 weeks when stored at 2 to 8 °C in the dark. Do not freeze.
Note: Do not store in plastic as adsorption of ingredients to the plastic surface may occur.
Altre note
For life science research only. Not for use in diagnostic procedures.
Note legali
Nutridoma is a trademark of Roche
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
No data available
Punto d’infiammabilità (°C)
No data available
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