P7181
Polyethylene glycol solution
Hybri-Max™, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma
Sinonimo/i:
PEG Solution, Polyethylene Glycol
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About This Item
Prodotti consigliati
Grado
Hybri-Max™
Livello qualitativo
Sterilità
0.2 μm filtered
Nome Commerciale
BioReagent
Stato
solution
PM
average mol wt 1,450
Concentrazione
50 % (w/v)
tecniche
cell culture | hybridoma: suitable
Impurezze
endotoxin, tested
Temperatura di conservazione
2-8°C
Stringa SMILE
C(CO)O
InChI
1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
LYCAIKOWRPUZTN-UHFFFAOYSA-N
Categorie correlate
Descrizione generale
Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.
Applicazioni
Polyethylene glycol solution has been used in the hybridization of the following:
- irradiated camel fibroblast cells with A23 hamster cells
- mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells
- rabbit splenocytes and cell line 240E-1
Recommended for use in a normal fusion protocol requiring 50% PEG.
PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.
PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.
Azioni biochim/fisiol
Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics. PEG is commonly used as a lubricant. It′s application is also observed in chromatographic stationary phases. PEG prevents crystal formation in emulsions.
Confezionamento
Packaged in sealed ampules under nitrogen.
Altre note
Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.
Ricostituzione
Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.
Note legali
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
212.0 °F - closed cup
Punto d’infiammabilità (°C)
100 °C - closed cup
Dispositivi di protezione individuale
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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