SR-4987

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94102704, mouse bone, Fibroblast-like

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: mouse bone
• Confezionamento: tube of 5 μg 94102704-DNA-5UG; pkg of vial of cells 94102704-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Fibroblast-like
• Prodotti: Macrophage colony stimulating factor (M-MCF)
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse bone marrow stromal cells

Descrizione della linea cellulare

A bone marrow-derived stromal cell line established by A Pessina in 1987 from a normal female 5 month old mouse. A bone marrow culture was treated with the supernatant from Y1 cells which actively produce viral C-particles (MuLV). This fibroblast-like cell line produces macrophage-colony stimulating factor (M-MCF) and exhibits a high sensitivity to fibroblast growth factor (FGF) in clonogenic assays. The cells produce sarcomas in syngenic mice and have a reported doubling time of 14.7 hours.

Terreno di coltura

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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