FGC4

94101904, rat skin, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: FGC4, 94101904
• Origine biologica: rat skin
• Modalità di accrescimento: Adherent
• Cariotipo: Modal no. 52
• Morfologia: Epithelial
• Prodotti: Albumin and ß-fibrogen
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat hepatoma [alb-gpt plasmid]

Descrizione della linea cellulare

A subclone derived from Fao which is deficient in HGPRT (resistant to 5μg/ml 6-thioguanine and also resistant to 3mM ouabain). FGC4 contains the alb-gpt plasmid which is the bacterial gpt gene under control of the rat albumin promoter (400 bp). Produces albumin and ß-fibrinogen.GMO1

Applicazioni

Gene expression studies.

Terreno di coltura

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split semi-confluent cultures (70%) 1:100 i.e. seeding at 2x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change three times a week.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.