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Key Documents

GE29-0915-98

Sigma-Aldrich

Superose® 6 Increase

3.2/300, Cytiva 29-0915-98, 8.6 μm avg. part. size

Sinonimo/i:

Size exclusion chromatography, Superose 6 column

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About This Item

Codice UNSPSC:
41115700
NACRES:
SB.52

Materiali

glass column

Nome Commerciale

Superose®

PM

5000-5000000 Da (globular proteins)

Produttore/marchio commerciale

Cytiva 29-0915-98

Parametri

<0.03 mL/min flow rate (20% ethanol at low temp)
<0.07 mL/min flow rate (20% ethanol at room temp)
<0.07 mL/min flow rate (water at low temp)
<0.15 mL/min flow rate (water at room temp)
3 Mpa (435 psi)
4-50 μL sample volume

Matrice

cross-linked agarose
polymeric

Gruppo funzionale matrice

agarose phase

Dimens. media delle particelle

8.6 μm

cleaning

1-14

working range

3-12

Tecnica di separazione

size exclusion (SEC)

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Categorie correlate

Descrizione generale

Superose® 6 Increase 3.2/300 is a versatile, prepacked column for size exclusion chromatography in small-scale preparative purification (4 to 50 μl sample volume), as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000. When high resolution is required, sample amount is limited and low buffer consumption is important, this is the column of choice.

Caratteristiche e vantaggi

  • Increased resolution compared with Superose 6, for higher purity and improved analysis results.
  • Reduced runtime compared with Superose 6, for rapid results.
  • Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
  • Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

Compatibilità

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)

Stoccaggio e stabilità

Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Note legali

Superdex is a registered trademark of Cytiva
Superose is a registered trademark of Cytiva

Codice della classe di stoccaggio

3 - Flammable liquids

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Protocolli

Performing a Separation with Superose

Superose from GE Healthcare are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

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