HTC

93120108

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: rat liver
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 66, hypertriploid
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Dexamethasone phosphate; hydrocortisone
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat hepatoma

Descrizione della linea cellulare

Derived from a rat ascites tumour, which in turn was derived from a solid hepatoma induced in male Buffalo rats by feeding them 0.04% N,N′-2,7fluorenyle-bis-2,2,2-trifluoroacetamide for 12.4 months.

Applicazioni

Tumourigenicity and enzymatic studies

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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