LS180

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87021202, human colon, Epithelial-like

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human colon
• Confezionamento: tube of 5 μg 87021202-DNA-5UG; pkg of vial of cells 87021202-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Modal no. 45 (2n=46)
• Morfologia: Epithelial-like
• Prodotti: Carcinoembryonic antigen (CEA), Mucin
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian colon adenocarcinoma

Descrizione della linea cellulare

Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon. The cells secrete CEA and mucin and are tumorigenic in nude mice. LS174T (Sigma Catalogue number. 87060401) was derived from the same patient. The cells have not been exposed to trypsin.

Applicazioni

Tumourigenicity studies

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² by scraping; 5% CO₂; 37°C.

Altre note

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