A549 Cell Line human

86012804, human lung (carcinoma), Epithelial

• Sinonimo/i: A-549 Cells, A549 Cells, NCI-A549 Cells
• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: A549 Cell Line human, 86012804, from human lung(carcinoma)
• Origine biologica: human lung (carcinoma)
• Modalità di accrescimento: Adherent
• Cariotipo: Hypotriploid
• Morfologia: Epithelial
• Prodotti: Lecithin, High amounts of de-saturated fatty acids.
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian lung carcinoma

Descrizione della linea cellulare

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

Applicazioni

Enzymatic studies

Human A549 cell line has been used:

• to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions
• to study the effects of insulin and insulin-like growth factor 1 (IGF1) on proliferation and apoptosis in A549 cells
• to culture A549 cells in different media over a long term and identify conditions for promoting alveolar type II differentiation characteristics in A549 cells

Profilo DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14

Terreno di coltura

Ham′s F12K + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or tTrypsin/EDTA; 5% CO₂; 37°C.

Altre note

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