SH-SY5Y Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94030304, human nerve, Neuroblast

• Sinonimo/i: SHSY5Y Cells, SK-SH-SY5Y Cells, SY5Y Cells
• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human nerve
• Confezionamento: tube of 5 μg 94030304-DNA-5UG; pkg of vial of cells 94030304-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Neuroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: metastasis
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human neuroblastoma

Descrizione della linea cellulare

SH-SY5Y is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH (Sigma catalogue no. 86012802). SH-SY-5Y has dopamine-β-hydroxylase activity and can convert glutamate to the neurotransmitter GABA. Will form tumours in nude mice in approximately 3-4 weeks. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as noradrenalin uptake or neuronal markers routinely.

Applicazioni

SH-SY5Y cell line human is used for neurotransmitter studies
SH-SY5Y cell line human has been used to:

• study the iron-mediated toxicity of amyloid β peptide
• check the significance of anionic luminescent conjugated polythiophene (LCP) polythiophene acetic acid (PTAA) in vital staining of cells
• study the effect of haloperidol on sigma-1 receptors in guinea pig brain and human neuroblastoma cells

Confezionamento

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Altre note

• DNA is stored at -70 °C
• RNA is stored at -70 °C
• Cell line is stored at -196 °C

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Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18

Terreno di coltura

Ham′s F12:EMEM (EBSS) (1:1) + 2mM Glutamine + 1% Non Essential Amino AcidsNEAA + 15% Foetal Bovine Serum FBS / FCS.

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:10 to 1:100 i.e. seeding at 1x1,000-1x10,000 cells/cm²; using 0.25% Trypsin or Trypsin/EDTA, 5% CO₂; 37°C. Cells may reattach slowly and may remain in suspension for several days. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as noradrenalin uptake or neuronal markers routinely.