Passa al contenuto
Merck
Tutte le immagini(2)

Key Documents

View All Documentation

C9658

Sigma-Aldrich

Carboxypeptidase G from Pseudomonas sp.

lyophilized powder, ≥3 units/mg protein

Sinonimo/i:

γ-Glutamyl hydrolase

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(2)

About This Item

Numero CAS:
9074-87-7
Classificazione EC (Enzyme Commission):
3.4.17.11 (BRENDA, IUBMB)
Numero CE:
232-978-1
Numero MDL:
MFCD00130719
Codice UNSPSC:
12352204
NACRES:
NA.54

Forma fisica

lyophilized powder

Livello qualitativo

300

Attività specifica

≥3 units/mg protein

PM

homodimer ~90 kDa

Composizione

Protein, ~70% biuret

Solubilità

H2O: soluble 0.5 mg/mL

Temperatura di conservazione

−20°C

Cerchi prodotti simili? Visita Guida al confronto tra prodotti

Applicazioni

Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells.

Azioni biochim/fisiol

Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.

Definizione di unità

One unit will hydrolyze 1.0 μmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.

Stato fisico

Contains sodium acetate salt.

Nota sulla preparazione

Chromatographically purified
Solutions should be prepared fresh prior to use.

Pittogrammi

Health hazardExclamation mark
GHS08,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H315,H319,H334,H335

Classi di pericolo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Jeremy P Mallari et al.
Bioorganic & medicinal chemistry, 12(22), 6011-6020 (2004-10-23)
A series of alkyl and aryl phosphonyl, thiophosphonyl, and dithiophosphonyl derivatives of (S)- and (R)-glutamic acid were prepared and examined for inhibitory potency against glutamate carboxypeptidase (carboxypeptidase G). The acquisition of the phosphonamidodithioic acids and the individual phosphonamidothioic acid diastereomers
Takashi Tsukamoto et al.
Bioorganic & medicinal chemistry letters, 12(16), 2189-2192 (2002-07-20)
Phosphonate and phosphinate analogues of N-acylated gamma-glutamylglutamate were tested for the ability to inhibit glutamate carboxypeptidase II (GCP II). All of the compounds inhibit GCP II with IC(50) values in the low nanomolar range. The comparison of the results to
Masaharu Suzuki et al.
Current opinion in plant biology, 11(5), 548-553 (2008-08-12)
Two subfamilies of plant-specific B3 domain transcription factors regulate the fundamental transition between seed and vegetative phases of development. The AFL B3 genes activate the embryo maturation program, while the closely related VAL B3 genes shutdown the AFL network before
Edgar M Pera et al.
Gene expression patterns : GEP, 3(2), 147-152 (2003-04-25)
Endoderm development is an area of intense interest in developmental biology, but progress has been hampered by the lack of specific markers for differentiated endodermal cells. In an unbiased secretion cloning screen of Xenopus gastrula embryos we isolated a novel
The enzymatic hydrolysis of methotrexate and folic acid.
C C Levy et al.
The Journal of biological chemistry, 242(12), 2933-2938 (1967-06-25)
Visualizza tutti i documenti correlati

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.