C9243

Anticorpo anti-CHIP (regione C-terminale)

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Anti-CHIP, carboxy terminus of Hsp70p-interacting protein, Anti-HSPABP2, Anti-NY-CO-7, Anti-STUB1, Anti-UBOX1
• Codice UNSPSC: 12352203
• NACRES: NA.41
• Coniugato: unconjugated
• application: WB
• Clone: polyclonal
• Reattività contro le specie: human, mouse, rat
• citations: 18
• tecniche: western blot: 1-2 μg/test using HEK-293T cells lysate and mouse brain extract (S2 fraction)

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Stato: buffered aqueous solution
• PM: antigen ~35 kDa
• Reattività contro le specie: human, mouse, rat
• Concentrazione: ~1 mg/mL
• tecniche: western blot: 1-2 μg/test using HEK-293T cells lysate and mouse brain extract (S2 fraction)
• N° accesso UniProt: Q9UNE7
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... STUB1(10273) ; mouse ... Stub1(56424) ; rat ... Stub1(287155)

Descrizione

Descrizione generale

CHIP (carboxy terminus of Hsp70-interacting protein, also known as STIP1-homology and U-box containing protein 1, STUB1, HSPABP2, NY-CO-7, SDCCAG7, UBOX1), is a dual-function chaperone/E3 ubiquitin ligase. CHIP has 3 functional domains: a N-terminal tetratricopeptide repeat (TPR), a U-box at its C-terminus, and a highly charged internal region. It is located on human chromosome 16p13.3.

Applicazioni

Anti-CHIP (C-terminal) antibody produced in rabbit has been used in western blotting and histology to stain testes and ovary sections.

Anti-CHIP (C-terminal) antibody produced in rabbit is suitable for immunoblotting at a working concentration of 1-2 μg/mL using HEK-293T cells lysate and mouse brain extract (S2 fraction).

Azioni biochim/fisiol

CHIP (carboxy terminus of Hsp70-interacting protein) plays a crucial role in regulating protein quality control at multiple levels. It ubiquitinates Hsp70, a cytosolic chaperone and enhances refolding of stress damaged proteins. Additionally, it has E3 ubiquitin ligase activity and triggers proteasome degradation of irreversibly damaged proteins to prevent cellular toxicity. The ubiquitination, aggregation and degradation of several proteins involved in neurodegenerative disorders including tau, huntingtin, Cu/Zn SOD1, ataxin-1, and α-synuclein are regulated by CHIP and Hsp70. The aggregation and toxicity of polyglutamine (polyQ) expanded proteins is suppressed by CHIP. It also regulates stress-dependent apoptosis.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)