C3766
Cholesterol Esterase from bovine pancreas
lyophilized powder, ≥200 units/g protein
Sinonimo/i:
Sterol-ester acylhydrolase
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Prodotti consigliati
Forma fisica
lyophilized powder
Livello qualitativo
Attività specifica
≥200 units/g protein
PM
84 kDa by SDS-PAGE
Composizione
protein, ≥20%
Temperatura di conservazione
−20°C
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
Descrizione generale
Cholesterol Esterase from bovine pancreas is a member of α-β hydrolase superfamily. The core of the protein comprises 11 β-strands, surrounded by 15 α-strands. Catalytic triad is in the centre of the molecule. The C-terminus possesses hydrophobic residues.
Applicazioni
Cholesterol Esterase from bovine pancreas has been used:
- in γ-oryzanol-hydrolyzing activity
- in cholesterol esterase enzyme activity for quantification of cholesterol and cholesterol esters associated in tear film and lenses samples
- to test in vitro degradation of poly(fumaroyl bioxirane) maleate (PFM) polymer designed for bone tissue engineering
Cholesterol esterase bound to membrane-associated heparin on brush border membranes aids in the transport of cholesterol and free fatty acid across the membrane. This enzyme is widely used in the determination of serum cholesterol in clinical laboratories. The enzyme from Sigma has been used to evaluate the inhibitory and antioxidant functions of the methanol extract of the Camellia sinensis leaves under in vitro conditions. The enzyme has also been used to digest human serum samples to confirm the presence and position of acyl esters of 7α-hydroxycholesterol.
Enzyme responsible for the hydrolysis of many of the fatty acid esters of cholesterol.
Optimum pH range: 6-8
Activators: Bisphenol A diglycidyl ether, cAMP-dependent protein kinase type II, ethanol, methanol, n-butanol, phosphatidylcholine, phosphatidylethanolamine, sodium taurocholic acid
Inhibitors: Bisphenol A methacrylate, diisopropylfluorophosphate, enolase, Hg2+, sodium fluoride, phosphatidic acid, phosphatidylcholine, phosphatidylserine
Optimum pH range: 6-8
Activators: Bisphenol A diglycidyl ether, cAMP-dependent protein kinase type II, ethanol, methanol, n-butanol, phosphatidylcholine, phosphatidylethanolamine, sodium taurocholic acid
Inhibitors: Bisphenol A methacrylate, diisopropylfluorophosphate, enolase, Hg2+, sodium fluoride, phosphatidic acid, phosphatidylcholine, phosphatidylserine
Azioni biochim/fisiol
Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. This monomeric glycoprotein may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis. Its molecular mass is found to be 84 kDa.
Altre note
Partially purified
Definizione di unità
One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per minute at pH 7.0 at 37 °C in the presence of taurocholate.
Stato fisico
This product is partially purified from bovine pancreas and is supplied as a white to light brown lyophilized powder containing ≥20% protein (biuret) and potassium phosphate.
Nota sulla preparazione
Cholesterol esterase is soluble in 0.4 M potassium phosphate, pH 7.0 at 1 mg/mL concentration.
Risultati analitici
Protein determined by biuret.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Enzymatic quantification of cholesterol and cholesterol esters from silicone hydrogel contact lenses.
Pucker AD, et al.
Investigative Ophthalmology & Visual Science, 51(6), 2949-2954 (2010)
H Oda et al.
Journal of lipid research, 31(12), 2209-2218 (1990-12-01)
Serum levels of 7 alpha-hydroxycholesterol and activities of hepatic microsomal cholesterol 7 alpha-hydroxylase in surgical patients were analyzed by capillary gas-liquid chromatography-selected ion monitoring technique using a new internal standard, 5 alpha-cholestane-3 beta, 7 beta-diol. We found that concentrations of
Enzyme-catalyzed hydrolysis of gamma-oryzanol.
Miller A, et al.
European Food Research and Technology, 218(4), 349-354 (2004)
A Lopez-Candales et al.
Biochemistry, 32(45), 12085-12089 (1993-11-16)
We have recently hypothesized that neutral lipids can, in part, move across biological membranes via a mechanism involving enzymes anchored to membrane proteoglycans such as those found in the brush border of the enterocyte [Bosner, M. S., Gulick, T., Riley
A functional polymer designed for bone tissue engineering.
You Z, et al.
Acta Biomaterialia, 8(2), 502-510 (2012)
Articoli
Cholesterol undergoes esterification to improve transport. Cholesterol esters are more easily packaged into the interior of lipoproteins - increasing the quantity that can be readily transported in the blood stream.
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.