C1403
Cholesterol Esterase from Pseudomonas sp.
lyophilized powder, ≥200,000 units/g protein
Sinonimo/i:
Cholesterol Esterase from Pseudomonas fluorescens, Sterol-ester acylhydrolase
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About This Item
Prodotti consigliati
Stato
lyophilized powder
Livello qualitativo
Attività specifica
≥200,000 units/g protein
PM
~300 kDa
Composizione
protein, ≥40% biuret
Temperatura di conservazione
−20°C
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Applicazioni
This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.
Azioni biochim/fisiol
Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The enzyme may have multiple functions in lipid and lipoprotein metabolism, and atherosclerosis.
Proprietà fisiche
Stability: Stable at –20°C for at least one year
Isoelectric point: 5.9 ± 0.1
Michaelis constants: 5.4 x 10‾5M (Linoleate), 6.6 x 10‾5M (Oleate)
3.7 x 10‾5M (Linolenate), 1.5 x 10‾4M (Palmitate)
1.2 x 10‾4M (Myristate), 2.3 x 10‾5M (Stearate)
Inhibitors: Hg++, Ag+, ionic detergents
Optimum pH: 7.0 − 9.0
Optimum temp: 40°C
pH Stability: pH 5.0 − 9.0 (25°C, 24hr)
Thermal stability: Below 55°C (pH 7.5, 10min)
Isoelectric point: 5.9 ± 0.1
Michaelis constants: 5.4 x 10‾5M (Linoleate), 6.6 x 10‾5M (Oleate)
3.7 x 10‾5M (Linolenate), 1.5 x 10‾4M (Palmitate)
1.2 x 10‾4M (Myristate), 2.3 x 10‾5M (Stearate)
Inhibitors: Hg++, Ag+, ionic detergents
Optimum pH: 7.0 − 9.0
Optimum temp: 40°C
pH Stability: pH 5.0 − 9.0 (25°C, 24hr)
Thermal stability: Below 55°C (pH 7.5, 10min)
Definizione di unità
One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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Articoli
Cholesterol undergoes esterification to improve transport. Cholesterol esters are more easily packaged into the interior of lipoproteins - increasing the quantity that can be readily transported in the blood stream.
Protocolli
Protocol for Assay Procedure for Cholesterol Esterase
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