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L3126

Sigma-Aldrich

Lipase from porcine pancreas

Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)

Sinonimo/i:

Pancreatic lipase, PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase

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About This Item

Numero CAS:
9001-62-1
Classificazione EC (Enzyme Commission):
3.1.1.3 (BRENDA, IUBMB)
Numero CE:
232-619-9
Numero MDL:
MFCD00131509
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

Porcine pancreas

Livello qualitativo

200

Tipo

Type II

Forma fisica

lyophilized (cake)

Attività specifica

≥125 units/mg protein (using olive oil (30 min incubation))
30-90 units/mg protein (using triacetin)

Composizione

protein, ≥20%

Temperatura di conservazione

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
QWZUIMCIEOCSJF-CHHCPSLASA-N

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Applicazioni

Lipase from porcine pancreas has been used:
  • for the analysis of fatty acid at the 2-position of triglycerides.
  • to determine the indigestible fraction of plant source that is resistant to the action of digestive enzymes.
  • as a component of dissolution media to study the dissolution profiles of theophylline.
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Azioni biochim/fisiol

Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Qualità

Crude (Steapsin). Contains amylase and protease activity.

Definizione di unità

One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)

Risultati analitici

Protein determined by biuret.

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Jiawei Wan et al.
Food chemistry, 221, 760-770 (2016-12-17)
Soy protein isolate (SPI) was selected to fabricate supersaturated self-emulsifying nanoemulsions, aiming to enhance physical stability and bioaccessibility of hydrophobic tangeretin. Dissolution studies demonstrated that tangeretin had the highest solubility in Tween 80, followed by oil phase solutions, and polymer
M T Andonaegui et al.
Drug development and industrial pharmacy, 25(11), 1199-1203 (1999-12-22)
Dissolution profiles of theophylline (TP) from three types of sustained-release (SR) matrix tablets (plastic [PL], lipid [LP], and hydrophilic [HP]) in different dissolution media, with and without enzymes, were established. Also investigated was the influence of a treatment of the
The contribution of fruits and vegetables to dietary intake of polyphenols and antioxidant capacity in a Mexican rural diet: Importance of fruit and vegetable variety.
Hervert-Hernandez D et al.
Food Research International, 44, 1182-1189 (2011)
Enzymatic transesterification of rapeseed oil and lauric acid in a continuous reactor.
Forssell, P., et al.
Journal of the American Oil Chemists' Society, 70, 1105-1109 (1993)
Peiliang Zhang et al.
Journal of pharmaceutical and biomedical analysis, 174, 486-494 (2019-06-24)
Natural compounds with diverse structural skeletons have different ionization efficiencies and different mass spectrometry (MS) responses. Some key factors influencing the electrostatic field induced spray ionization (EFISI)-MS for a variety of natural compounds have been optimized and improved. Fifteen reference
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