Passa al contenuto
Merck
Tutte le immagini(3)

Key Documents

Altri documenti

C0543

Sigma-Aldrich

Rat Collagen Type IV

from Engelbreth-Holm-Swarm murine sarcoma basement membrane, lyophilized powder, suitable for cell culture, used for 3D hydrogel formation

Sinonimo/i:

Type IV collagen, EHS-collagen

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(3)

About This Item

Numero CAS:
9007-34-5
Numero CE:
232-697-4
Numero MDL:
MFCD00130825
Codice UNSPSC:
12352202
NACRES:
NA.75

product name

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture

Origine biologica

mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)

Livello qualitativo

200

Tipo

Type IV (Miller)

Nome Commerciale

BioReagent

Forma fisica

lyophilized powder

Confezionamento

glass bottle of 1 vial

tecniche

cell culture | mammalian: suitable

Copertura della superficie

6‑10 μg/cm2

Solubilità

0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)

N° accesso UniProt

B1AVK5
P02463
P08122
Q63ZW6
Q9QZS0

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Informazioni sul gene

mouse ... Col4a1(12826) , Col4a2(12827) , Col4a3(12828) , Col4a5(12830) , Col4a6(94216)

Descrizione generale

Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Applicazioni

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used:
  • in adhesion assays.
  • in hydrogel fabrication.
  • to coat cell culture plates for cell differentiation.
  • to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells.
Recommended for use as a cell culture substratum at 6-10 μg/cm2.

Azioni biochim/fisiol

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.

Altre note

Native collagen
We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types.

Quantità

0.75 mg/vial

Nota sulla preparazione

Chloroform treated.
Pepsin is not used in the preparation.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Slide 1 of 4

1 of 4

Sigma-Aldrich

Sigma-Aldrich

C8919

Collagene

Collagene Bornstein and Traub Type I, powder, BioReagent, suitable for cell culture

Sigma-Aldrich

C7661

Collagene

Sigma-Aldrich

Sigma-Aldrich

C2249

Collagen Solution from human fibroblasts

Sigma-Aldrich

Sigma-Aldrich

C3867

Collagene tipo I

Evaluation of cell binding activities of Leptospira ECM adhesins.
Robbins G T, et al.
PLoS Neglected Tropical Diseases, 9(4), e0003712-e0003712 (2015)
Gels Handbook: Fundamentals, Properties, Applications null
Mimicking the Extracellular Matrix: The Intersection of Matrix Biology and Biomaterials (2015)
Cell Motility in Cancer Invasion and Metastasis (2006)
X Li et al.
The Journal of cell biology, 153(4), 811-822 (2001-05-16)
Fibroblast growth factors and receptors are intimately connected to the extracellular matrix by their affinity to heparan sulfate proteoglycans. They mediate multiple processes during embryonic development and adult life. In this study, embryonic stem cell-derived embryoid bodies were used to
Visualizza tutti i documenti correlati

Articoli

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.