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Documenti fondamentali

A9210

Sigma-Aldrich

Annexin V FITC Conjugate from human placenta

Sinonimo/i:

Annexin V, Calphosbindin I, Lipocortin V, PAP-1

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About This Item

Codice UNSPSC:
41106305
NACRES:
NA.32

Stato

buffered aqueous solution

Livello qualitativo

Grado di funzionalizzazione

1-2 mol FITC per mol annexin V

N° accesso UniProt

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Informazioni sul gene

human ... ANXA5(308)

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Applicazioni

For detection of apoptotic cells by flow cytometry or fluorescence microscopy.

Azioni biochim/fisiol

Annexins are ubiquitous homologous proteins that bind phospholipids in the presence of calcium.
Annexins are ubiquitous homologous proteins that bind phospholipids in the presence of calcium. The cellular changes involved in the apoptotic process include loss of phospholipid asymmetry during the early stages. This phenomenon is universal and is not limited to stimulus or to mammalian cells, but also occurs in insect and plant cells. In living cells, phosphatidylserine is transported to the inside of the lipid bilayer by the Mg2+ATP dependent enzyme, aminophospholipid translocase. At the onset of apoptosis, phosphatidylserine becomes translocated to the external surface of the cell membrane. Since the movement of phosphatidylserine from the internal membrane surface to the external surface is an early indicator of apoptosis, annexin V and its conjugates that interact strongly and specifically with phosphatidylserine may be used to detect apoptosis. Annexin V conjugates can be used to detect apoptotic cells significantly earlier than DNA-based assays. Fluorescent dye labeled annexin V have applications in flow cytometry, fluorescence microscopy, and laser scanning cytometry.

Confezionamento

Package size based on protein content

Stato fisico

Solution in 50 mM Tris-HCl, pH 7.5, containing 100 mM NaCl.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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