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94281

Sigma-Aldrich

Urease from Canavalia ensiformis (Jack bean)

powder, white, ~8 U/mg

Sinonimo/i:

Jack bean urease, Urea amidohydrolase

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About This Item

Numero CAS:
9002-13-5
Classificazione EC (Enzyme Commission):
3.5.1.5 (BRENDA, IUBMB)
Numero CE:
232-656-0
Numero MDL:
MFCD00070858
Codice UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54

Origine biologica

Canavalia ensiformis

Livello qualitativo

100

Forma fisica

powder

Attività specifica

~8 U/mg

PM

Mr ~480000

Colore

white

Temperatura di conservazione

−20°C

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Descrizione generale

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH. Urease is present in archaea, bacteria, unicellular eukaryotes and plants.

Applicazioni

Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes . It may be useful for the detection of pathogens as well as heavy-metal ions.

Azioni biochim/fisiol

Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit .

Definizione di unità

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol urea (Cat. No. 51459) per minute at pH 8.0 and 25°C

Pittogrammi

Health hazardExclamation mark
GHS08,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H315,H319,H334,H335

Classi di pericolo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificati d'analisi (COA)

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