65497

TRIS acetate-EDTA Buffer solution

for Northern and Southern blotting, solution

• Codice UNSPSC: 12161700

Proprietà

• Grado: Molecular Biology; for molecular biology
• Nome Commerciale: BioReagent
• Stato: solution
• Impurezze: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• pH: 8.3±0.2
• Densità: 1.00 g/mL at 20 °C
• Temperatura di conservazione: room temp

Descrizione

Descrizione generale

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but dsDNA runs faster with TAE buffer. This concentrate can be easily diluted to 0.5x before use (with molecular biology grade water).

Applicazioni

Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. Especially useful for dsDNA separations.

Componenti

The TAE liquid buffer is a 1x solution that contains 0.04M TRIS acetate and 0.001M EDTA in water.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves