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Documenti fondamentali

52526

Sigma-Aldrich

Anti-Human IgG (whole molecule)−Atto 488 antibody produced in goat

~1 mg/mL

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

goat

Coniugato

Atto 488 conjugate

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

contiene

50% glycerol as stabilizer

Reattività contro le specie

human

Concentrazione

~1 mg/mL

Fluorescenza

λex 500 nm; λem 522 nm in PBS

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Categorie correlate

Descrizione generale

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders,. Atto 488-goat anti-human IgGs are known to associate with human IgGs.

Immunogeno

human IgG

Applicazioni

Atto 488-goat anti-human IgG may be used for fluorescence-based immunoassays.

Stato fisico

Atto 488 goat anti-human IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.

Risultati analitici

unconjugated dye ≤5% of total fluorescence; dye-to protein ration ≥2

Note legali

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Certificati d'analisi (COA)

Lot/Batch Number

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W Wisemandle et al.
Pediatrics, 106(1), E14-E14 (2000-07-06)
To determine the effects of timing of onset of overweight (OW) on childhood anthropometric adiposity levels using long-term serial data from the Fels Longitudinal Study. OW was defined as body mass index (BMI; kg/m(2)) >25, and the age at first
Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving.
Bretschneider, S.; Eggeling, Chr.; Hell, S. W.
Physical Review Letters, 98(21) (2007)
F Cataldo et al.
Minerva pediatrica, 42(12), 509-514 (1990-12-01)
IgG are the most common isotype of Ig and include four subclasses which differ from one another in the following ways: their initial amino acid sequence, their physical and chemical properties and the different serum concentrations reached with age. Every
C Papadea et al.
Critical reviews in clinical laboratory sciences, 27(1), 27-58 (1989-01-01)
Human IgG consists of two identical heavy (H) chains and two identical light (L) chains joined by interchain disulfide bridges. Heterogeneity in the amino acid sequences of the H and L polypeptides results in at least three types of IgG
Dual color localization microscopy of cellular nanostructures.
Gunkel, M.; et al.
Biotechnology Journal, 4(6), 927-938 (2009)

Articoli

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

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