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Key Documents

18772

Sigma-Aldrich

Anti-Rabbit IgG - Atto 488 antibody produced in goat

whole molecule, 1 mg/mL protein

Sinonimo/i:

Atto 488-[goat-Anti-rabbit IgG], whole molecule

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.46

Coniugato

Atto 488 conjugate

Livello qualitativo

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

contiene

50% glycerol

Reattività contro le specie

rabbit

Concentrazione

1 mg/mL protein

tecniche

immunocytochemistry: 1:500
immunofluorescence: 1:200
immunohistochemistry: suitable

Fluorescenza

λex 485 nm; λem 552 nm in PBS

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Goat polyclonal anti-Rabbit IgG (whole molecule)-Atto 488 antibody antibody reats with rabbit IgG in vitro, in rabbit serum and biological fluids.
Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Atto 488 is a labeling dye with high molecular absorption (90,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. It is optimized for excitation with an argon laser, and is characterized by high photostability. Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Immunogeno

Rabbit IgG

Applicazioni

Anti-Rabbit IgG has been used for immunocytochemistry applications at a dilution of 1:500 in neural precursor cells.
Goat polyclonal anti-Rabbit IgG (whole molecule)-Atto 488 antibody may be used to detect and quantitate the level of IgG in rabbit serum and biological fluids via fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use rabbit IgG as the primary antibody. Atto 488 goat anti-rabbit IgG antibody was used for immunofluorescence to detect L. monocytogenes in mouse tissue sections following incubation with an anti-listeria antibody. Antibody was used at a 1:200 dilution.

Stato fisico

Atto 488 goat anti-rabbit IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.

Risultati analitici

Unconjugated dye ≤5% of total fluorescence; dye-to-protein ratio >2.

Note legali

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Tatiana D Mayorova et al.
Scientific reports, 11(1), 23343-23343 (2021-12-04)
Placozoa is a phylum of non-bilaterian marine animals. These small, flat organisms adhere to the substrate via their densely ciliated ventral epithelium, which mediates mucociliary locomotion and nutrient uptake. They have only six morphological cell types, including one, fiber cells
Lin Wang et al.
Oncology letters, 23(5), 149-149 (2022-03-31)
Throughout the world, numerous individuals are infected with Toxoplasma gondii, which may improve immunity against cancer. Furthermore, microRNAs (miRs) may be differentially expressed in the host upon infection with T. gondii. In the present study, RNA-sequencing analysis and reverse transcription-quantitative
Javier A Carrero et al.
Infection and immunity, 77(10), 4371-4382 (2009-08-12)
Listeriolysin O (LLO) is an essential virulence factor for the gram-positive bacterium Listeria monocytogenes. Our goal was to determine if altering the topology of LLO would alter the virulence and toxicity of L. monocytogenes in vivo. A recombinant strain was
Linjie Pan et al.
Journal of neuroscience research, 87(14), 3207-3220 (2009-06-17)
The traditional notion that injured neurons are unable to regenerate in the adult mammalian brain and spinal cord has long been a concern. This view has led to methodology designed to overcome this problem, most recently by advancements in tissue
Janin Lautenschläger et al.
Nature communications, 9(1), 712-712 (2018-02-21)
Alpha-synuclein is known to bind to small unilamellar vesicles (SUVs) via its N terminus, which forms an amphipathic alpha-helix upon membrane interaction. Here we show that calcium binds to the C terminus of alpha-synuclein, therewith increasing its lipid-binding capacity. Using

Articoli

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

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