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F1262

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

goat

Coniugato

FITC conjugate

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Stato

buffered aqueous solution

tecniche

indirect immunofluorescence: 1:40

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Goat polyclonal anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–FITC antibody binds all rabbit IgGs.
Immunoglobulins (Igs) are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs can be useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule), (F(ab′)2) fragment-FITC is specific for all rabbit immunoglobulins. The use of this product prevents background staining due to the presence of Fc receptors.
Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.

Immunogeno

Purified rabbit IgG

Applicazioni

Anti-Rabbit IgG (whole molecule), (F(ab′)2) fragment-FITC is suitable for use in flow cytometry.
Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–FITC antibody produced in goat has also been used for fluorescence labeling and immunocytochemistry.
Goat polyclonal anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–FITC antibody may be used to detect and quantitate the level of IgG in rabbut serum and biological fluids by fluorescent techniques. It may also be used as a secondary antibody in assays that use rabbit IgG as the primary antibody. F(ab′)2 fragment–FITC antibody is useful when trying to avoid background staining due to the presence of Fc receptors.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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