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Documenti fondamentali

41658

Sigma-Aldrich

Lipase A Candida antarctica immobilized on Immobead 150, recombinant

≥500 U/g

Sinonimo/i:

Candida antarctica Lipase

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About This Item

Codice UNSPSC:
12352204
NACRES:
NA.54

Ricombinante

expressed in Aspergillus oryzae

Stato

beads (powder like)
beads

Attività specifica

≥500 U/g

Temperatura di conservazione

2-8°C

Categorie correlate

Descrizione generale

Research area: Cellsignalling. Lipase A Candida Antarctica, CalA is a thermostable, calcium-dependent enzyme with high substrate specificity. CalA comprises of the catalytic triad (Ser184, Asp334, His366) and has an α/β hydrolase structural fold.

Applicazioni

Lipase A Candida antarctica immobilized on Immobead 150, recombinant from Aspergillus oryzae has been used in the synthesis of enantiopure (R)-salsolinol and adsorption kinetics studies using Quartz crystal microbalance with dissipation (QCM-D).It has also been used tostudy the esterification of difluorinated alcohols.
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Azioni biochim/fisiol

Lipase A Candida Antarctica, CalA is highly specific for alcohols and esterifies the trans-isomer of fatty acids. CalA recognizes highly branched acyl groups and is active on alcohols with steric hindrance. CalA catalyzes the production of enantiopure amino acids and aids in the synthesis of chiral cyanohydrins. It may find industrial applications for its thermostable functionality in paper industry.Lipase A Candida antarctica(CAL-A) shows acetyltransferase activity by synthesizing fatty acid esters from certain alcohols and natural oils in an aqueous environment.

Definizione di unità

1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 10.0 and 40°C (tributyrin, Cat. No. 91010, as substrate)

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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Improved acylation of phytosterols catalyzed by Candida antarctica lipase A with superior catalytic activity
Panpipat W, et al.
Biochemical Engineering Journal, 70(1), 55-62 (2013)
Lipase-catalyzed synthesis of the chiral tetrahydroisoquinoline (R)-salsolinol
Ding W, et al.
Tetrahedron, 23(18-19), 1376-1379 (2012)
Orientating lipase molecules through surface chemical control for enhanced activity: a QCM-D and ToF-SIMS investigation
Joyce P, et al.
Colloids and Surfaces. B, Biointerfaces, 142, 173-181 (2016)
High yield expression of Lipase A from Candida antarctica in the methylotrophic yeast Pichia pastoris and its purification and characterisation
Pfeffer J, et al.
Applied Microbiology and Biotechnology, 72(5), 931-931 (2006)
Biotechnological applications of Candida antarctica lipase A: State-of-the-art
de Maria PD, et al.
Journal of Molecular Catalysis. B, Enzymatic, 37(1-6), 36-46 (2005)

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