62288

Lipase B Candida antarctica, recombinant from Aspergillus oryzae

powder, beige, ~9 U/mg

• Numero CAS: 9001-62-1
• Classificazione EC (Enzyme Commission): 3.1.1.3 (BRENDA, IUBMB)
• Numero CE: 232-619-9
• Numero MDL: MFCD00131509
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Ricombinante: expressed in Aspergillus oryzae
• Livello qualitativo: 100
• Forma fisica: powder
• Attività specifica: ~9 U/mg
• PM: 33 kDa
• Colore: beige
• Temperatura di conservazione: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;; QWZUIMCIEOCSJF-CHHCPSLASA-N

Descrizione

Descrizione generale

Candida antarctica lipase B (CalB) is structurally similar to several other lipases and has a flexible lid. It is made up of 317 amino acids and has a molecular weight of 33 kDa. Lipase B is a member of the alpha/beta hydrolase-fold family.

Applicazioni

Lipase B Candida antarctica, recombinant from Aspergillus oryzae has been used:

• as a standard to characterize the enzymatic properties of D5-CalB
• as an efficient biocatalyst to start the reaction to obtain (R)-ester via esterification of racemic secondary alcohol
• to investigate a “green” recycling route for polybutylene succinate (PBS) based on reactive extrusion
• to compare the esterification yield with adsorbed CaLB (aCaLB) and covalently immobilized CaLB (cCaLB)

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Azioni biochim/fisiol

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Candida antarctica lipase B (CALB) possesses wide substrate specificity, high activity and high enantioselectivity, hence it is considered as a major enzyme in biotechnology. It also has the capability to perform in aqueous and non-aqueous reaction environments. CALB is used in transesterification, kinetic resolution and polymerization reactions.

Lipase B from Candida antarctica has been shown to be an effective catalyst for the synthesis of esters of ethyl D-glucopyranoside from fatty acids larger than octanoic acid. It has also been found to catalyze a wide variety of organic reactions including many different regio- and enantio-selective syntheses.

Definizione di unità

1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 8.0 and 40°C (tributyrin, Cat. No. 91010, as substrate)

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H334
• Consigli di prudenza: P261 - P284 - P501
• Classi di pericolo: Resp. Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)