09111802

MS-1 cell line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 09111802, human skin, mesothelioma. Loose aggregates with irregular outline, no central necrosis.

• Sinonimo/i: MS1 Cells
• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human skin
• Stato: liquid
• Confezionamento: tube of 5 μg 09111802-DNA-5UG; pkg of vial of cells 09111802-1VL
• Modalità di accrescimento: Suspension
• Cariotipo: Not determined
• Morfologia: Loosely packed floating aggregates with irregular outline and no central necrosis
• Prodotti: Cell line is CM2B4/MCV T, AE1/AE2, CK20, EMA, CAM5.2, chromogranin & NSE. Cell line is negative for for CK7, synaptophysin, LCA TTF-1, vimentin and CD99,
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer

Descrizione

Origine della linea cellulare

Human neuroendocrine skin carcinoma

Descrizione della linea cellulare

MS-1 cells contain a Merkel cell polyomavirus integrated into the genome. See also MKL-1 (Sigma Catalogue number 09111801).

Applicazioni

Pathobiology of neuroendocrine skin carcinomas (NSCs) and function of cutaneous Merkel cells. The role of polyomavirus in the development of Merkel cell carcinomas (submitted for publication)

Confezionamento

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Profilo DNA

STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11,14
D16S539: 9
D5S818: 11
D7S820: 8,9
THO1: 7,9.3
TPOX: 8
vWA: 17

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Seed at 3 x 10⁵ cells/ml in 5% CO₂; 37°C Saturation density is approximately 10⁶ cells/ml.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr Patrick Moore, University of Pittsburgh Cancer Institute, 5117 Centre Avenue Suite 1.8 Pittsburgh PA 15213

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable