03978

Methylene Blue solution

for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

• Sinonimo/i: Loeffler’s Methylene Blue
• Formula empirica (notazione di Hill): C₁₆H₁₈ClN₃S
• Numero CAS: 61-73-4
• Peso molecolare: 319.85
• Beilstein: 3641570
• Numero MDL: MFCD00012111
• Codice UNSPSC: 12171500
• ID PubChem: 329747853
• NACRES: NA.47

Proprietà

• Grado: for microscopy
• Livello qualitativo: 100
• Forma fisica: liquid
• Qualità: concentrated, aqueous solution
• Durata: ~3 yr
• Concentrazione: 1.5% (1.5 g/ 100 mL)
• Densità: 1.0 g/mL at 20 °C
• εmax: 9.4 at 656-666 nm in water
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: room temp
• Stringa SMILE: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1; CXKWCBBOMKCUKX-UHFFFAOYSA-M

Descrizione

Descrizione generale

Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.

Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.

Applicazioni

Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.

Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter