PPB015

Tris-Glycine Buffer

pH 8.3, pHast Pack^™, powder

• Sinonimo/i: 1X TG buffer, TG, TG Buffer, Tris Glycine running buffer, Tris Glycine transfer buffer, Tris glycine
• Codice UNSPSC: 41105319
• NACRES: NA.25

Proprietà

• Nome Commerciale: pHast Pack^™
• Livello qualitativo: 100
• Forma fisica: powder
• Condizioni di stoccaggio: (Tightly closed. Dry)
• Impurezze: ≤5 ppm Heavy Metals (lead)
• Colore: white to off-white
• pH: 8.3
• Solubilità: soluble, clear, colorless to faintly yellow
• Cationi in tracce: Fe: <10 ppm
• Attività estranea: DNase, none detected; Endonuclease ; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• Temperatura di conservazione: room temp

Descrizione

Descrizione generale

Tris-Glycine buffer is primarily used as a running buffer and to prepare transfer buffer in western blot analysis with the addition of methanol. This buffer can separate proteins by their size. It has a pH of 8.3.

Applicazioni

Suitable for use as:

• Transfer buffer component in western blot
• Running buffer in native gel electrophoresis for protein separation
• Running buffer in gradient polyacrylamide gel electrophoresis (PAGE)

Caratteristiche e vantaggi

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Saves time and minimizes the effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Confezionamento

Foil pouches

Ricostituzione

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 25 mM Tris, 192 mM Glycine, pH 8.3 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

Stoccaggio e stabilità

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Altre note

For additional information on our range of Biochemicals, please complete this form.

Note legali

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1