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Key Documents

F5415

Sigma-Aldrich

Ficoll® solution

Type 400, 20% in H2O

Sinonimo/i:

Ready-to-use density gradient media

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About This Item

Numero CAS:
26873-85-8
Numero MDL:
MFCD00081599
Codice UNSPSC:
12352207
NACRES:
NA.75

Sterilità

0.2 μm filtered

Livello qualitativo

200

Tipo

Type 400

Forma fisica

liquid

Concentrazione

20% in H2O

Colore

clear colorless

Solubilità

water: soluble

Densità

1.055-1.075 g/mL

applicazioni

hematology
histology

Temperatura di conservazione

2-8°C

Categorie correlate

Descrizione generale

Ficoll® Solution is a highly branched polymer of sucrose, also known as polydisperse mixtures of dextran. Ficoll® molecules are inert and not re-absorbed by proximal tubules. The structure is open and deformable, and not a rigid sphere thereby becoming a preferred choice in “crowding” experiments.
Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.

Applicazioni

Ficoll® has been used in


  • Isolation of cord blood mononuclear cells
  • Isolation of CD133 negative cells
  • Isolation and expansion of circulating endothelial progenitor cells.

Altre note

A nonionic synthetic polymer of sucrose.

Note legali

Ficoll is a registered trademark of Cytiva

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
SLCL2553
SLCJ9378
SLCH6817
SLCH3511
SLCH0230

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Addensante Histopaque®-1077 Hybri-Max™

Xiaoliang Wu et al.
Shock (Augusta, Ga.), 51(6), 718-724 (2018-08-07)
The aim of this study was to investigate the correlation between plasma sphingosine-1-phosphate (S1P) and ceramide concentrations in sepsis, and the possible mechanisms for altered expression. Plasma S1P and ceramide concentrations were measured by HPLC-ESI-MS/MS. HLA-DR (human leukocyte antigen-DR) expression
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The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have
Effect of mononuclear cells on morphometric of kidney parameters in hyperglycemic rat.
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Annals of Experimental Biology, 2(3), 1-7 (2014)
Analysis of Structural and Functional Glomerular Changes in Hyperglycemic Rats that Treated with Human Umbilical Cord Blood Derived CD133 Negative Ccells.
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Protocolli

Hematopoietic Stem Cell Culture Protocols

Step-by-step hematopoietic stem cell culture protocols for isolation, expansion and differentiation of CD34+ hematopoietic progenitor cells including CFU assays. It can be cultured under defined conditions designed either to promote self-renewal and increase the number of primitive cells.

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