GE17-5446-02
Ficoll®-Paque PREMIUM 1.084
Cytiva 17-5446-02, pack of 6 × 100 mL
Sinonimo/i:
Ficoll PREMIUM
About This Item
Prodotti consigliati
Forma fisica
liquid
Confezionamento
pack of 6 × 100 mL
Produttore/marchio commerciale
Cytiva 17-5446-02
Colore
Colorless to slightly yellow
Intervallo di pH
5.5-7.5
Descrizione generale
Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2003.
Applicazioni
Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.
Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).
Caratteristiche e vantaggi
- Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
- Manufactured under a Quality Management System certified to ISO 13485:2003.
- Low levels of endotoxin (< 0.12 EU/mL) secured and tested.
- Cytotoxicity grade 0.
- A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.
Stoccaggio e stabilità
Risultati analitici
Note legali
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Articoli
Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads as an external marker facilitates monitoring of the gradient shape and range.
The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.
This page shows methods to remove Percoll from a sample after centrifugation.
The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.
Protocolli
How to use percoll to prepare a gradient
The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.
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