67996

Tris Acetate-EDTA buffer Concentrate

for Northern and Southern blotting, solution

• Codice UNSPSC: 12161700
• NACRES: NA.25

Proprietà

• Grado: Molecular Biology; for molecular biology
• Stato: solution
• Qualità: 50x Concentrate
• Impurezze: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• pH: 8.3±0.2 (25 °C)
• Densità: 1.09 g/mL at 20 °C
• Temperatura di conservazione: room temp

Descrizione

Descrizione generale

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double stranded DNA runs faster with TAE buffer. This 50x concentrate can be easily diluted with molecular biology grade water to 1x before use.

Applicazioni

Tris Acetate-EDTA buffer concentrate is suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. It is especially useful for double stranded DNA separations.

Componenti

The TAE 50x liquid concentrate contains 2M TRIS acetate and 0.05M EDTA in water.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable