49940

ONPG Disks

suitable for microbiology

• Sinonimo/i: β-Galactosidase Test Disks, 2-Nitrophenyl-β-D-galactopyranoside Disks
• Codice UNSPSC: 41171621
• NACRES: NA.85

Proprietà

• agenzia: according to GB 4789.4-2016
• Nome Commerciale: BioChemika
• Durata: limited shelf life, expiry date on the label
• tecniche: microbe id | specific enzyme detection: suitable
• applicazioni: environmental; food and beverages; microbiology
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• Compatibilità: Escherichia coli; bacteria

Descrizione

Descrizione generale

ONPG Disks are used to detect the presence of β-galactosidase, an enzyme found in lactose-fermenting organisms. Lactose utilization depends upon two enzymes: β-galactoside permease, which catalyzes transport of lactose into the cell, and β-galactosidase, which breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides including the ONPG (o-nitrophenyl-β-D-galactopyranose) substrate. ONPG hydrolysis results in the release of galactose, and the yellow chromogenic compound, o-nitrophenol. The test substrate, ONPG, does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period even for late lactose fermenters.

Confezionamento

Sterile filter paper discs (50 pcs, diameter 6 mm) impregnated with o-nitrophenyl-β-D-galactopyranose

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)