COLLH-RO

Collagenase H

from Clostridium histolyticum

• Classificazione EC (Enzyme Commission): 3.4.24.3 (BRENDA, IUBMB)
• Codice UNSPSC: 12352204

Proprietà

• Origine biologica: Clostridium histolyticum
• Livello qualitativo: 100
• Sterilità: non-sterile
• Forma fisica: lyophilized
• collagenase activity: 0.15 U/mg lyophilizate
• Confezionamento: pkg of 100 mg (11074032001); pkg of 2.5 g (11087789001); pkg of 500 mg (11074059001)
• Produttore/marchio commerciale: Roche
• Concentrazione: ~1 mg/mL (Isolation of rat hepatocytes); ~2 mg/mL (Isolation of adipocytes)
• tecniche: tissue processing: suitable
• Colore: brown
• pH ottimale: 6.0-8.0
• Solubilità: water: soluble
• N° accesso NCBI: WP_138210331
• N° accesso UniProt: Q46085
• applicazioni: life science and biopharma
• Attività estranea: Clostripain 39.798 U/mg; Proteases 14.014 U/mg (Azocoll, based on lyoph); Trypsin 1.668 U/mg (with BAEE)
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Collagenase H is an enzyme mixture prepared from Clostridium histolyticum cultures by filtration, ammonium sulfate precipitation, dialysis, and lyophilization.

Specificità

Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases also degrade other proteins.

Applicazioni

Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Collagenase H is well suited for the isolation of hepatocytes from rat liver by the collagenase perfusion method. This enzyme preparation is also suitable for the preparation of other types of cells such as endothelial cells from large vessels, and for the isolation of adipocytes from epididymal fat pads of rats.

Stato fisico

Lyophilizate, nonsterile

Nota sulla preparazione

Enzyme activity:
Collagenase activity: >0.15U/mg (according to Wünsch) (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate).
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase H has a balanced ratio of enzyme activities, and is function tested for the isolation of rat hepatocytes (perfusion method).
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Activator: Ca²⁺
Working concentration: 0.5 to 2.5mg/ml,
Approximately 1 mg/ml for the isolation of rat hepatocytes and 2mg/ml for the isolation of adipocytes.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution should be stored at -15 to -25°C for short term storage, -60°C or as described below for long-term storage.

Ricostituzione

Reconstitution in any balanced salt solution (e.g., HBSS)

Stoccaggio e stabilità

Keep container tightly closed in a dry and well-ventilated place.

Altre note

For life science research only. Not for use in diagnostic procedures.

Informazioni sulla sicurezza

• Pittogrammi: GHS07,GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H315 - H319 - H334 - H335
• Consigli di prudenza: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Classi di pericolo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash