11276905001
Roche
Interferon-γ, mouse (mIFN-γ)
recombinant (E. coli)
Sinonimo/i:
mIFN
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Ricombinante
expressed in E. coli
Saggio
>95% (SDS-PAGE)
Forma fisica
solution
Attività specifica
>5*10^6 U/mg
Confezionamento
pkg of 100,000 U (20 μg, 1 ml)
Produttore/marchio commerciale
Roche
Temperatura di conservazione
−20°C
Categorie correlate
Descrizione generale
(mIFN- γ , NIH, reference standard, Gg 02–901-533, at least the same specific activity (EC 50 ) compared to the indicated standard is guaranteed.
IFN-γ (interferon-γ) is produced by natural killer T cells, macrophages, Th1 CD4 T cells and activated CD8 T cells and the production increases with age.
Recombinant, Interferon-γ, mouse (mlFN-γ) is produced in E. coli and purified by standard chromatographic techniques.
Primary structure: One polypeptide chain (131 amino acids) identical to mouse, natural IFN- ? (133 amino acids), but lacking the last two amino acids at the carboxy-terminus and not glycosylated. Glycosylation is not essential for biological activity.
Primary structure: One polypeptide chain (131 amino acids) identical to mouse, natural IFN- ? (133 amino acids), but lacking the last two amino acids at the carboxy-terminus and not glycosylated. Glycosylation is not essential for biological activity.
Specificità
Species specificity: mouse
Applicazioni
Interferon-γ, mouse (mIFN-γ) has been used in cell culture.
Recombinant mouse interferon-γ is a valuable tool for the study of IFN-γ (interferon-γ) actions in mouse model systems.
Azioni biochim/fisiol
Interferon-γ (IFNγ) participates in cell-mediated immunity and has pro-inflammatory effects on immunocytes and target tissue. Its functions vary and is tissue specific. IFNγ induces T-cell-mediated immune response by increasing MHC-I expression on target cells and acts through IFNγ receptor, which is expressed on both immune and non-immune cells. IFNγ is associated with the development of colitis and Sjögren Syndrome.
Qualità
- Purity: >95% (SDS-PAGE)
- Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
Sequenza
Chain Length 131 AA
The primary structure of recombinant Interferon-γ, mouse (mIFN-γ) is identical to that of natural, mouse IFN-γ (one polypeptide chain, 131 amino acids), however, recombinant mIFN-γ lacks the last two amino acids at the C-terminus and is not glycosylated. Glycosylation is not essential for biological activity.
Definizione di unità
EC 50 definition : The amount of mIFN- γ that is required to produce equivalent antiviral activity to that expressed by 1 unit of the NIH IFN-γ reference standard (Gg 02–901-533) (L cells-EMC virus/cytopathic effect) (1 unit equals =0.2 ng/ml).
EC50 definition: One unit is defined as the amount of mlFN-γ that is required to produce equivalent antiviral activity to that expressed by 1 unit of the NIH IFN-γ reference standard (Gg 02-901-533). (L cells-EMC virus/cytopathic effect) (1 unit equals ≤0.2 ng/ml).
Stato fisico
Solution, filtered through 0.2 μm pore size membrane, 100 000 U/ml (20 μg/ml) in PBS (phosphate buffered saline) and BSA (bovine serum albumin),1 mg/ml [purity of BSA: 98%, endotoxin (LAL): < 1 EU/mg BSA].
Nota sulla preparazione
Working solution: Dilute the concentrated mlFN-γ solution (100,000 U/ml) with PBS or culture medium containing BSA, 1 mg/ml (0.1%) or serum, 1 to 10%.
Storage conditions (working solution): -15 to -25 °C
Store the solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Storage conditions (working solution): -15 to -25 °C
Store the solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Altre note
For life science research only. Not for use in diagnostic procedures.
For life science research only. Not for use in diagnostic procedures.
1 EU corresponds to 0.1 ng.
1 EU corresponds to 0.1 ng.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Interferon-gamma deficiency protects against aging-related goblet cell loss.
Oncotarget, 7(40), 64605-64605 (2016)
The Rac-specific exchange factors Dock1 and Dock5 are dispensable for the establishment of the glomerular filtration barrier in vivo.
Small Gtpases, 4(4), 221-230 (2013)
Interferon-? derived from cytotoxic lymphocytes directly enhances their motility and cytotoxicity.
Cell Death & Disease, 8(6), e2836-e2836 (2017)
The mTORC2/Akt/NF?B pathway-mediated activation of TRPC6 participates in adriamycin-induced podocyte apoptosis.
Cellular Physiology and Biochemistry, 40(5), 1079-1093 (2016)
Bio-protocol, 10(15) (2020-11-20)
Studying monocytic cells in isolated systems in vitro contributes significantly to the understanding of innate immune physiology. Functional assays produce read outs which can be used to measure responses to selected stimuli, such as pathogen exposure, antigen loading, and cytokine
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.