MABS443

Anti-GRP78 Antibody, clone C38

clone C38, from mouse

• Sinonimo/i: 78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: C38, monoclonal
• Reattività contro le specie: human
• tecniche: flow cytometry: suitable
• Isotipo: IgG2bκ
• N° accesso NCBI: NP_005338
• N° accesso UniProt: P11021
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... HSPA5(3309)

Descrizione

Descrizione generale

The gene HSPA5 encodes a protein named GRP-78 (78 kDa glucose-regulated protein) also known as BiP (immunoglobulin heavy chain-binding protein) or heat shock 70 kDa protein 5. While originally discovered as one of set of genes that were induced upon cellular starvation and stress, HSPA5 encoded GRP-78 is now known to be an important member of the heat-shock family of proteins. The HSPA5 encoded GRP-78 protein appears to play a role in helping in the assembly of multimeric protein complexes in the endoplasmic reticulum (ER). Cellular localization is in the endoplasmic reticulum lumen and appears as cytoplasmic-like staining. The HSPA5 encoded protein is an essential component of the translocation processes within the endoplasmic reticulum and also functions in the retrograde transport across the ER membrane of incorrectly folded proteins destined to be degraded by the ubiquitin proteasome system. The HSPA5 encoded protein, while widely expressed in all cells types under normal conditions, is particularly induced under conditions of cellular stress, heat, or toxins and conditions which lead to the accumulation of unfolded proteins in the ER.

Immunogeno

Recombinant protein corresponding to human GRP78.

Applicazioni

Anti-GRP78 Antibody, clone C38 detects level of GRP78 & has been published & validated for use in GRP78.

Flow Cytometry Analysis: A representative lot from an independent laboratory detected GRP78 in HepG2 cells (de Ridder, G. G., et al. (2012). Melanoma Res. 22(3):225-235.).

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Qualità

Evaluated by Flow Cytometry in HeLa cells.

Flow Cytometry Analysis: 1 µg of this antibody detected GRP78 in 1X10E6 HeLa cells.

Descrizione del bersaglio

72 kDa calculated

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable