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Merck
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Documenti fondamentali

MAB2170

Sigma-Aldrich

Anti-Huntingtin Disease (HD/HTT) Antibody

CHEMICON®, mouse monoclonal, HU-4E6

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Nome del prodotto

Anti-Huntingtin Protein Antibody, a.a. 1247-1646, clone HU-4E6, ascites fluid, clone HU-4E6, Chemicon®

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

ascites fluid

Tipo di anticorpo

primary antibodies

Clone

HU-4E6, monoclonal

Reattività contro le specie

mouse, human

Produttore/marchio commerciale

Chemicon®

tecniche

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG2b

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

Specificità

Huntingtin Protein. No detectable cross reactivity with other proteins by Western blot.

Immunogeno

Epitope: a.a. 1247-1646
Huntingtin fragment from aa 1247 to 1646 as a fusion protein

Applicazioni

Detect Huntingtin Protein using this Anti-Huntingtin Protein Antibody, a.a. 1247-1646, clone HU-4E6 validated for use in ELISA, IP, WB, IC, IH(P).
ELISA: 1:500-1:5,000

Western blot (recombinant protein, not suitable for the natural Huntingtin) : 1:500-1:5,000

Immunohistochemistry on frozen and microwave oven treated paraffin sections (human): 1:500-1:5,000

Immunocytochemistry on transfected cells: 1:500-1:5,000

Immunoprecipitation: 1:500-1:5,000

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Stato fisico

Ascites fluid. Liquid, does not contain any preservative.

Stoccaggio e stabilità

Maintain at -20°C in undiluted aliquots up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Raccomandato

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Molecular cloning and characterization of the common marmoset huntingtin gene.
Hirohiko Hohjoh,Hirofumi Akari,Yuko Fujiwara,Yoshiko Tamura,Hirohisa Hirai,Keiji Wada
Gene null
Neurons lacking huntingtin differentially colonize brain and survive in chimeric mice.
A Reiner, N Del Mar, C A Meade, H Yang, I Dragatsis, S Zeitlin, D Goldowitz
The Journal of Neuroscience null
Anjalika Chongtham et al.
Human molecular genetics, 29(4), 674-688 (2020-01-17)
Huntington's disease (HD) is caused by an expansion of a poly glutamine (polyQ) stretch in the huntingtin protein (HTT) that is necessary to cause pathology and formation of HTT aggregates. Here we ask whether expanded polyQ is sufficient to cause
Huntingtin modulates transcription, occupies gene promoters in vivo, and binds directly to DNA in a polyglutamine-dependent manner.
Benn, CL; Sun, T; Sadri-Vakili, G; McFarland, KN; DiRocco, DP; Yohrling, GJ; Clark et al.
The Journal of Neuroscience null
F R Fusco et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 19(4), 1189-1202 (1999-02-10)
Immunohistochemistry and single-cell RT-PCR were used to characterize the localization of huntingtin and/or its mRNA in the major types of striatal neurons and in corticostriatal projection neurons in rats. Single-label immunohistochemical studies revealed that striatum contains scattered large neurons rich

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