MAB13446

Anti-TIMP-2 Antibody, clone 2TMP05

clone 2TMP05, Chemicon^®, from mouse

• Sinonimo/i: Tissue Inhibitor of Metalloproteinase-2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 2TMP05, monoclonal
• Reattività contro le specie: human, mouse, bovine, guinea pig, rabbit, rat
• Produttore/marchio commerciale: Chemicon^®
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• N° accesso NCBI: NM_003255.4
• N° accesso UniProt: P16035
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... TIMP2(7077)

Descrizione

Specificità

MAB13446 recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinase-2 (TIMP-2). TIMP-2 is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. CHEMICON MAB13446 may be used to evaluate the invasive nature of some tumor types and determine the tissue distribution of TIMPs in other tumors such as adenomas and also its normal tissue.

CELLULAR LOCALIZATION:

Cytoplasmic

Immunogeno

A synthetic peptide from the N-terminal region of human TIMP-2 protein.

Applicazioni

Detect TIMP-2 using this Anti-TIMP-2 Antibody, clone 2TMP05 validated for use in WB, IH(P).

Immunohistology (formalin/paraffin only): 2-4μg/ml 30 min at RT.

Western blotting: 1μg/ml for 2 hrs at RT.

(Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min. followed by cooling at RT for 20 min).

The optimal dilution for a specific application under a given set of experimental conditions should be determined by the investigator.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Linkage

Replaces: MAB13448

Stato fisico

Format: Purified

Purified from ascites fluid by Protein A chromatography. 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Stoccaggio e stabilità

Antibody with sodium azide is stable for 12 months after date of receipt when stored at 2-8°C.

Risultati analitici

Control
POSITIVE CONTROL:

Pancreatic or colon carcinoma

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable