MAB1120
Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31
clone 24-31, Chemicon®, from mouse
Sinonimo/i:
IGF-IR, CD221
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
24-31, monoclonal
Reattività contro le specie
human
Produttore/marchio commerciale
Chemicon®
tecniche
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
Isotipo
IgG1
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... IGF1R(3480)
Specificità
Recognizes the alpha-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R). It shows no cross-reaction with insulin receptor. Its epitope localizes between a.a. 283-440 (exon 4-6) of IGF-1R. Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The a-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. It is assumed that, as for the insulin receptor, the tyrosine kinase activity is essential for IGF-1 action.
Weakly reacts with Rabbit.
Weakly reacts with Rabbit.
Immunogeno
IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA.
Applicazioni
Acts as a Weak IGF-like Agonist in Bioassays (Thymidine incorporation into DNA)
Immunoprecipitation (Use Protein G): 2μg antibody/mg protein lysate.
Immunohistochemistry (Frozen and formalin/paraffin): Use antibody at 2-4ug/mL for 30 min. at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min. followed by cooling at room temperature for 20 minutes. Please note that EDTA is better than citrate for epitope unmasking.
Optimal working dilutions must be determined by end user.
Immunoprecipitation (Use Protein G): 2μg antibody/mg protein lysate.
Immunohistochemistry (Frozen and formalin/paraffin): Use antibody at 2-4ug/mL for 30 min. at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min. followed by cooling at room temperature for 20 minutes. Please note that EDTA is better than citrate for epitope unmasking.
Optimal working dilutions must be determined by end user.
Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31 detects level of Insulin-like Growth Factor-1 Receptor & has been published & validated for use in IH(P) & IP.
Research Category
Signaling
Signaling
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
Stato fisico
Format: Purified
Purified from ascites fluid by Protein G chromatography. Liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Stoccaggio e stabilità
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
M A Soos et al.
The Biochemical journal, 290 ( Pt 2), 419-426 (1993-03-01)
Hybrid insulin/insulin-like growth factor-I (IGF-I) receptors have previously been described in human placenta, but it has not been possible to study their properties in the presence of classical insulin receptors and type I IGF receptors. To facilitate the purification of
Expression of the IGF system in human adrenal tissues from early infancy to late puberty: implications for the development of adrenarche.
Baquedano, MS; Berensztein, E; Saraco, N; Dorn, GV; de Davila, MT; Rivarola, MA; Belgorosky, A
Pediatric Research null
Esperanza B Berensztein et al.
Pediatric research, 63(6), 662-666 (2008-06-04)
Immunoexpression of IGF-I, IGF-II, type 1 IGF receptor (IGFR), insulin receptor (IR), and GH receptor (GHR) was analyzed in human testis, in three age groups (Gr): Gr1 (neonates), Gr2 (postnatal testicular activation), and Gr3 (early prepuberty). In interstitial cells, low
Phase I/II trial and pharmacokinetic study of cixutumumab in pediatric patients with refractory solid tumors and Ewing sarcoma: a report from the Children's Oncology Group.
Malempati, S; Weigel, B; Ingle, AM; Ahern, CH; Carroll, JM; Roberts, CT; Reid et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology null
Low levels of insulin-like growth factor type 1 receptor expression at cancer cell membrane predict liver metastasis in Dukes' C human colorectal cancers.
Nakamura, M; Miyamoto, S; Maeda, H; Zhang, SC; Sangai, T; Ishii, G; Hasebe, T; Endoh et al.
Clinical cancer research : an official journal of the American Association for Cancer Research null
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