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Merck
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Key Documents

04-300

Sigma-Aldrich

Anti-phospho-Insulin Receptor (Tyr 1322) Antibody, clone 21G12

clone 21g12, Upstate®, from mouse

Sinonimo/i:

INSR

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

21g12, monoclonal

Reattività contro le specie

canine, human, mouse

Produttore/marchio commerciale

Upstate®

tecniche

ELISA: suitable
western blot: suitable

Isotipo

IgG1

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

phosphorylation (pTyr1322)

Informazioni sul gene

human ... INSR(3643)

Specificità

Recognizes phosphorylated domain of Insulin receptor on Tyrosine 1322.

Immunogeno

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Tyrosine 1322 on phosphorylated insulin receptor

Applicazioni

Anti-phospho-Insulin Receptor Antibody, clone 21G12 is an antibody against phospho-Insulin Receptor (Tyr 1322) for use in ELISA & WB.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

Qualità

Routinely evaluated by immunoblot.

Descrizione del bersaglio

97 kDa

Stato fisico

Format: Purified
Lyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose.
Subsequent thiophilic adsorption and size exclusion chromatography

Stoccaggio e stabilità

2 years at -20°C from date of shipment. Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 4°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Risultati analitici

Control
Includes pervanadate treated HEK293 lysate as a positive control.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids


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Molecular cloning of a DNA sequence complementary to creatine kinase M mRNA from chickens.
Schweinfest, CW; Kwiatkowski, RW; Dottin, RP
Proceedings of the National Academy of Sciences of the USA null
Frankie B Stentz et al.
Biochemical and biophysical research communications, 335(2), 491-495 (2005-08-09)
Upon activation by phytohemagglutine (PHA), T-lymphocytes (T-cells) express receptors for growth factors, insulin, IGF-1 and IL2 and become insulin sensitive. Diabetic ketoacidosis (DKA) is associated with in vivo emergence of these growth factor receptors without incubation with PHA. As DKA
Francesca Fiory et al.
Molecular and cellular biology, 25(24), 10803-10814 (2005-11-30)
In L6 myoblasts, insulin receptors with deletion of the C-terminal 43 amino acids (IR(Delta43)) exhibited normal autophosphorylation and IRS-1/2 tyrosine phosphorylation. The L6 cells expressing IR(Delta43) (L6(IRDelta43)) also showed no insulin effect on glucose uptake and glycogen synthase, accompanied by
Template to improve glycemic control without reducing adiposity or dietary fat.
Krishnapuram, R; Dhurandhar, EJ; Dubuisson, O; Kirk-Ballard, H; Bajpeyi, S; Butte et al.
American Journal of Physiology. Endocrinology and Metabolism null
Qiu-yan Wang et al.
The FEBS journal, 274(2), 526-538 (2007-01-19)
Two H7721 human hepatocarcinoma cell lines showing moderate and high expression of alpha1,3-fucosyltransferase (FucT)-VII cDNA were established and designated FucTVII-M and FucTVII-H, respectively. In alpha1,3-FucT-VII-transfected cells, expression of insulin receptor (InR) alpha- and beta subunits and epidermal growth factor receptor

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