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HPRTKMAG-01K

Millipore

MILLIPLEX® RTK Phosphoprotein Magnetic Bead Panel- Cell Signaling Multiplex Assay

allows simultaneous relative quantitation of multiple proteins in tissue and cell lysate samples

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About This Item

Codice UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Livello qualitativo

200

Reattività contro le specie

human

Produttore/marchio commerciale

Milliplex®

tecniche

multiplexing: suitable

Metodo di rivelazione

fluorometric (Luminex xMAP)

Condizioni di spedizione

ambient

Categorie correlate

Descrizione generale

RTKs (Receptor Tyrosine Kinases) are transmembrane proteins that act as receptors for growth factors, neurotrophic factors and other extracellular signaling molecules. Upon binding extracellular ligands, RTKs can undergo receptor dimerization and tyrosine phosphorylation on specific residues in the cytoplasmic tails which then lead to RTK-mediated signal transduction. Of the greater than 500 known protein kinases in human genome, approximately 60 are members of the RTK family. They are central components of cell signaling networks and play crucial roles in normal physiological processes and disease processes ranging from diabetes to cancer.

The MILLIPLEX® RTK Phosphoprotein Bead Customizable Panel is used to detect changes in phosphorylated (panTyr) c-Kit, c-Met/HGFR, EGFR, ErbB2/HER2, ErbB3/HER3 , ErbB4/HER4 , FGFR1 , FLT3 , IGF-1R, M-CSFR, PDGFRα, PDGFRβ, TIE1, TIE2, VEGFR1, VEGFR2, and VEGFR3 in cell lysates using the Luminex® system.

The MILLIPLEX® portfolio offers the broadest selection of analytes across a wide range of pathways. Once the panel of interest has been identified, you can rely on the quality we build into each kit to produce results you can trust. Performance criteria evaluated during the validation process include: cross-reactivity, assay CVs, kit stability, and sample behavior. In addition, each kit meets stringent Quality Control criteria to ensure lot-to-lot reproducibility.

The MILLIPLEX® RTK Phosphoprotein Bead Panel includes:


  • Positive and negative control cell lysates provided to qualify assay performance
  • Configurable beads to capture analytes of interest
  • Optimized detection antibody cocktail designed to yield consistent analyte profiles within a panel


Panel Type: Cell Signaling

Specificità

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Applicazioni

  • Analytes: c-Kit, c-Met/HGFR (panTyr), EGFR (pan Tyr), ErbB2/HER2, ErbB3/HER3 (panTyr) ,ErbB4/HER4 (panTyr), FLT3 (pan Tyr), IGF-1R (pan Tyr), M-CSFR (pan Tyr), PDGFR-α (pan Tyr), PDGFRß (pan Tyr), TIE1 (pan Tyr,) Tie2 (pan Tyr), VEGFR1 (pan Tyr), VEGFR2 (pan Tyr), VEGFR3 (pan Tyr), FGFR1
  • This 17-plex panel, unlike most signaling kits that are fixed/premixed, is customizable so that it is possible to choose only those analytes that are wanted or needed.
  • Recommended Sample type: cell lysate
  • Recommended starting concentration: 40 to 1,000 μg protein/mL
  • Assay Run Time: Overnight
  • Research Category: Signaling Apoptosis & Cancer

Caratteristiche e vantaggi

Design your multiplex kit by choosing available analytes within this panel.

Confezionamento

96-well plate

Stoccaggio e stabilità

Recommended storage for kit components is 2 - 8°C.

Note legali

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Avvertenze

Danger

Indicazioni di pericolo

H302,H315,H318,H410

Classi di pericolo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Adam Stewart et al.
Molecular cancer therapeutics, 18(8), 1396-1404 (2019-07-03)
It is increasingly appreciated that drug response to different cancers driven by the same oncogene is different and may relate to differences in rewiring of signal transduction. We aimed to study differences in dynamic signaling changes within mutant KRAS (KRAS

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Cell Signaling Multiplex Assays | MILLIPLEX® Assays

Uncover how cells communicate with MILLIPLEX® cell signaling multiplex assays. Multiplexing with cell signaling phosphoprotein assays based on Luminex® xMAP® technology helps researchers measure phosphoproteins and total proteins within the same or different pathways from a single sample.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.