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Documenti fondamentali

AB1526P

Sigma-Aldrich

Anti-Nerve Growth Factor-β Antibody, pain

Chemicon®, from rabbit

Sinonimo/i:

NGFbeta

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

affinity purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Purificato mediante

affinity chromatography

Reattività contro le specie

chicken, human, mouse, rat

Non deve reagire con

bovine

Produttore/marchio commerciale

Chemicon®

tecniche

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... NGF(4803)

Specificità

Nerve growth factor-beta (NGF). Less than 1% cross-reactivity against recombinant human BDNF, NT3 and NT4 by ELISA.

Immunogeno

Mouse nerve growth factor-beta purified from salivary glands.

Applicazioni

Immunohistochemistry: 1-2 μg/mL (see suggested protocol).

Immunoblotting: 1-2 μg/mL

Bioassays (Inhibition of biological activity in vitro): 1-2 μg/mL

in vivo animal studies: use neat

ELISA: 1-2 μg/mL

Optimal working dilutions must be determined by the end user.

BIOLOGICAL ACTIVITY:

This antibody completely inhibits neuron survival and the outgrowth actions of mouse NGF in chicken dorsal root ganglion in vitro.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
This Anti-Nerve Growth Factor-β Antibody, pain is validated for use in ELISA, WB, IH, FUNC for the detection of Nerve Growth Factor-β.

Linkage

Replaces: 04-1119

Stato fisico

Affinity purified immunoglobulin. Lyophilized from PBS. Contains no preservative. Reconstitute to desired volume with sterile distilled water. Suggested concentration 0.5-1.0 mg/mL.

Stoccaggio e stabilità

Maintain at lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Chronic nicotine administration increases NGF-like immunoreactivity in frontoparietal cerebral cortex.
R Martinez-Rodriguez, A Toledano, M I Alvarez, L Turegano, O Colman, P Roses et al.
Journal of Neuroscience Research null
Nerve growth factor of cultured medium extracted from human degenerative nucleus pulposus promotes sensory nerve growth and induces substance p in vitro.
Kazuyo Yamauchi,Gen Inoue,Takana Koshi,Masaomi Yamashita,Toshinori Ito,Munetaka Suzuki et al.
Spine null
T Nishio et al.
Neuroscience, 60(1), 67-84 (1994-05-01)
To elucidate the role and the mechanism of action of nerve growth factor in the adult central nervous system, we investigated the localization of nerve growth factor-like immunoreactivity in adult rat brain, both quantitatively and immunohistochemically, using polyclonal anti-nerve growth
Immunohistochemical distribution of NGF, BDNF, NT-3, and NT-4 in adult rhesus monkey brains.
Zhang, HT; Li, LY; Zou, XL; Song, XB; Hu, YL; Feng, ZT; Wang, TT
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society null
Biochemical effects of antibodies against nerve growth factor on developing and differentiated sympathetic ganglia.
M Goedert et al.
Brain research, 148(1), 264-268 (1978-06-09)

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