N5415
Nerve Growth Factor-ß human
recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Sinonimo/i:
β-NGF, BNGF, NGF-β, NGF-beta
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About This Item
Codice UNSPSC:
12352202
NACRES:
NA.77
Prodotti consigliati
Origine biologica
human
Livello qualitativo
Ricombinante
expressed in HEK 293 cells
Saggio
≥95% (SDS-PAGE)
Forma fisica
lyophilized powder
Potenza
≤16 ng/mL ED50/EC50
PM
protein calculated mol wt 13.5 kDa (Monomer)
Confezionamento
pkg of 10 μg
pkg of 100 μg
tecniche
cell culture | mammalian: suitable
Impurezze
≤1 EU/μg Endotoxin (β-NGF)
N° accesso UniProt
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... NGF(4803)
Categorie correlate
Descrizione generale
NGF-β gene is mapped to human chromosome 1p13.2.
Recombinant human β-NGF is expressed in human 293 cells as a homodimer (non-disulfide bonded) of 120 amino acids, with a calculated molecular weight of 13.5 kDa (monomer). Nerve growth factor β (β-NGF) is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. The production of natural β-NGF occurs in epithelial cells, and the pituitary gland.
Azioni biochim/fisiol
A mutation in the NGF-β gene is implicated in the loss of pain perception.
Nerve growth factor β (β-NGF) is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF promotes the survival and cholinergic phenotype of basal forebrain cholinergic neurons and stimulates neurite outgrowth of embryonic trigeminal motor neurons in vitro.
Avvertenza
The product retains activity for at least 2 years as supplied. After initial thawing it is recommended to store the protein in working aliquots at -20 °C.
Stato fisico
This product is lyophilized from PBS.
Nota sulla preparazione
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile DDW containing 0.1% endotoxin-free recombinant human serum albumin, to a final concentration of 100 μg/ml.
Stoccaggio e stabilità
Store product at –20 °C. The product retains activity for at least 2 years as supplied. After initial thawing it is recommended to store the protein in working aliquots at –20 °C.
Risultati analitici
The specific activity is determined by the dose dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Esclusione di responsabilità
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I clienti hanno visto anche
Yousong Su et al.
Progress in neuro-psychopharmacology & biological psychiatry, 104, 110015-110015 (2020-06-23)
The neurodevelopmental hypothesis of schizophrenia has been widely accepted. In light of our previous microarray data, two neurodevelopment-related genes were focused on inclduing the N-acylsphingosine amidohydrolase 1 gene (ASAH1) and the nerve growth factor gene (NGF). The evidence that ASAH1
T Lahtinen et al.
Neuroscience, 30(1), 165-170 (1989-01-01)
Explants of anterior, intermediate and posterior pituitary lobes of rats at various ages evoked an intense nerve fibre outgrowth when co-cultured with neonatal sympathetic superior cervical ganglia in a collagen gel medium. Freezing and thawing of pituitary lobes, prior to
Tissue localization of nerve growth factor and nerve growth factor receptors.
M Bothwell
Current topics in microbiology and immunology, 165, 55-70 (1991-01-01)
S Soinila et al.
Neuroscience research, 6(2), 123-130 (1988-12-01)
Nerve growth factor (NGF)-like immunoreactivity was demonstrated in the rat pituitary intermediate lobe by indirect immunofluorescence method using antisera specific to beta-NGF isolated from adult male mouse submaxillary salivary gland. Co-culture of frozen or fresh intermediate lobes with newborn rat
A Rosenthal et al.
Handbook of experimental pharmacology, 220, 497-512 (2014-03-29)
The neurotrophin family is comprised of the structurally related secreted proteins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophine-4 (NT-4). They bind and activate the tyrosine kinase receptors Trk A, B, and C in a ligand-specific
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